Proximal droplets in bovine sperm












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The solutions were prepared according to Celeghini et al. Abnormal morphology of bovine spermatozoa. Plasma membrane, acrosomal integrity and mitochondrial function were evaluated using the techniques described by Celeghini et al. However, as identification of specific abnormalities was an important consideration, each defect was counted separately, even if multiple defects occurred on the same sperm. At each examination, sperm were evaluated, and the morphology of each sperm was judged as either normal or abnormal.

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Breeding Soundness Exams Often Disrupted By Droplets

The solutions were prepared according to Celeghini et al. Sperm defects observed were grouped as follows. The objective of this study was to validate the assessment of bull sperm morphology by veterinary practitioners. S 87 The objective of this study was to investigate whether a higher percentage of spermatozoa with proximal cytoplasmic droplets could affect the quality and fertilising ability of frozen-thawed semen of Bos indicus Nellore breed bulls, using routine laboratory sperm assays, plasma membrane, acrosome and mitochondrial status, chromatin integrity and IVF. Table 3 Regression, correlation coefficients and levels of significance for proximal cytoplasmic droplets PCD versus sperm quality traits. Five bulls were selected for IVF.

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Roles of lipid peroxidation and cytoplasmic droplets on in vitro

However, the percentages of hatched blastocysts differed between bulls C1, C3 and P1 Evaluation of oocyte maturation conditions and bull effect on the in vitro bovine embryo production. Published online Jan Factors affecting breeding soundness classification of beef bulls examined at the Western College of Veterinary Medicine.

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Description: Oocyte collection and maturation Bovine ovaries were obtained in a local abattoir and transported to the laboratory. Abnormalities were designated as defects involving the head, midpiece, or tail, with variations among individual practitioners regarding more detailed characterization of sperm abnormalities. For each smear, cells were classified as follows. Bovine embryo development after IVF with spermatozoa having abnormal morphology. Individual semen characteristics were found not to affect cleavage but to affect embryo development [ 40 ]. Morphologically abnormal spermatozoa are more susceptible to oxidative stress. Evaluation of oocyte maturation conditions and bull effect on the in vitro bovine embryo production. The sperm morphology counts differed by no more than 20 percentage points for these bulls 98 times out of
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